Fig. 5.

Expression analyses in Gnetum gnemon.(A) Bisexual cone with a ring of ovules and rings of pollen cones developing basipetally at each node. (B to F) Ovules at different stages of development. (E) Inside of the mature ovule showing three different layers protecting the nucellus. (F) Fully mature seed. (G) Pollen cone with ovule at the top. (H to L) Developing ovules, note the expression of GneAP2 is restricted to the nucellus, integument, the middle envelop and later in the archegonia and pollen chamber. (M) Cross section of a young seed. Black arrowhead pointing to the megagametophyte; ar, archegonia; i, integument; me, middle envelop; ms, microsporangium; n, nucellus; o, ovule; oe, outer envelope; p, pollen; pc, pollen chamber; se, seed; t, tapetum. Scales: 50 µm (J toM), 100 µm (G to I), 0.5 cm (A to D), 2 cm (E and F).