Figure 4.

Differences in plaque SMC and collagen content in the aortic sinus in adult ${\text{apoE}}^{-/-}$ mice following early life arsenical exposure. ${\text{ApoE}}^{-/-}$ mice were exposed as described for Figure 1. (A–D) SMC and (E–H) collagen content were evaluated in the aortic sinus relative to the total lesion area using $\mathrm{\alpha }\text{-smooth}$ muscle actin and picrosirius red staining, respectively. Scale bar: $100\mathrm{\mu }\mathrm{m}$. (B) Represents the data distribution of the unadjusted percentage of SMCs with respect to the total sinus area for all offspring in the control and arsenical-exposed groups, whereas (C) and (D) represent that in the female and male offspring, respectively. (E) Represents the data distribution of the unadjusted percentage of collagen content with respect to the total sinus area for all offspring in the control and arsenical-exposed groups, whereas (F) and (G) represent that in the female and male offspring, respectively. The midline, box limits, whiskers, and dots denote the median, interquartile range, minimum and maximum values, and outliers, respectively. The corresponding numeric data are presented in Table S5 and that adjusted by litter are presented in Tables 4 and 5. Note: ${\text{DMA}}^{\mathrm{V}}$, cacodylic acid; ${\text{MMA}}^{\text{III}}$, monomethylarsonous acid; ${\text{MMA}}^{\mathrm{V}}$, disodium methyl arsonate hexahydrate; ${\text{NaAsO}}_2$, sodium arsenite; SMC, smooth muscle cell.