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. 2020 Nov 17;33(1):66–84. doi: 10.1093/plcell/koaa001

Figure 4.

Figure 4

Expression and subcellular localization of RH27 in Arabidopsis. (A–E) Expression of ProRH27:RH27-sYFP (yellow) in embryos at the zygote (A), 2-cell (B), 4-cell (C), globular (D), and torpedo-shaped (E) stages. Note the nuclear localization of the RH27-sYFP signals. Red, auto-fluorescence. Dashed lines outline the embryos. Bars = 20 µm for (A) to (D), and 50 µm for (E). (F) Expression of ProRH27:RH27-GUS (blue) in the shoot apex, specifically the SAM and leaf primordia (LP). The section was stained with periodic acid-Schiff (pink) to show the cellular structure. Bar = 50 µm. (G) Expression of ProRH27:RH27-sYFP (yellow) in the RAM. Part of the meristematic zone (framed) is magnified (right) to show the presence of RH27-sYFP in meristematic cells, but not the QC (arrowhead). The root was stained with propidium iodide (magenta) to show the cellular structure. Bars = 50 µm. (H) Subcellular localization of the RH27-sYFP protein (yellow) in a root epidermal cell from a transgenic plant carrying ProRH27:RH27-sYFP. The nucleus was stained with Hoechst 33258 (blue). Note that RH27-sYFP was localized primarily in the nucleolus (No) and to a small degree in the nucleoplasmic region (Np). Dotted lines outline the cell boundary. Bar = 5 µm.