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Image acquisition and volume-of-interest (VOI) segmentation. T2-weighted images (a, f, k), T2-weighted attenuated inversion recovery images (b, g, l), T1-weighted images (c, h, m), contrast-enhanced T1-weighted images (d, i, n) and tumor region (red in T2-weighted images) (e, j, o) show VOI segmentation in patients with grade II (a–e), grade III (f–j), and grade IV (k–o) glioma.
