Skip to main content
. 2021 May 20;16(5):e0251911. doi: 10.1371/journal.pone.0251911

Fig 4. CTNNB1 stabilization inhibits germ cell cluster-forming activity in vitro.

Fig 4

A) Representative photographs of germ cell clusters derived from RosaCtnnb1 and Rosa-control mice after 7 days of culture. B) Quantification of cluster formation from Thy1-positive cells derived from RosaCtnnb1 and Rosa-control mice (n = 6). The results indicate the number of clusters per 7.5 x 10^3 cells initially placed in culture. C) Cell death evaluation by propidium iodide incorporation in cultured spermatogonia derived from RosaCtnnb1 and Rosa-control mice (n = 3). D) Cell cycle profiles of cultured spermatogonia derived from RosaCtnnb1 and Rosa-control mice (n = 3). All data are expressed as mean (columns) ± SEM (error bars). Asterisks indicate significant differences from controls (*P < 0.05; **** P < 0.0001).