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. 2021 May 20;16(5):e0251911. doi: 10.1371/journal.pone.0251911

Fig 5. CTNNB1 stabilization skews cluster cell fate towards differentiation.

Fig 5

A) Evaluation of the number of KIT+ cells in Thy1-positive cell clusters derived from Rosa-ΔCtnnb1 mice and Rosa-control mice (n = 3). B) RT-qPCR analysis of the expression of genes involved in SSC maintenance or their transition to undifferentiated progenitor spermatogonia in clusters derived from Rosa-ΔCtnnb1 and Rosa-control mice (n = 10 for Rosa-control and n = 11 Rosa-ΔCtnnb1 for Gfra1, Plzf and Kit and n = 6 /genotype for Bcl6b, Rarg and Sohlh). RT-qPCR data were normalized to the housekeeping gene Rpl19. All data are expressed as mean (columns) ± SEM (error bars). Asterisks indicate significant differences from controls (**P < 0.01; *** P < 0.001; **** P < 0.0001).