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. 2021 May 1;49(9):4877–4890. doi: 10.1093/nar/gkab289

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Substrate scope: influence of substitution of the methylene amine of preQ₁. (A) Derivatives of preQ₁ with substitutions at the methylene amine. (B) Nucleobases (50 μM) were assessed for their ability to displace [³H] Guanine from pre-labeled tRNA^Tyr (10 μM) catalyzed by the QTRT enzyme (100 nM). Data are mean ± SD (n=3). Representative of two independent experiments.