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7-deazaguanine derivatives can be displaced from tRNA by QTRT. Evaluation of the ability of the QTRT enzyme (100 nM) to insert either; (A) [3H] guanine (200 nM) or (B) [3H] queuine (200 nM) into tRNA (10 μM) that had been pre-charged with natural or artificial nucleobases over a period of either 30 min or (C) 24 h. Data are mean ± SD (n=3). Representative of two independent experiments.
