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. 2021 May 3;49(9):5319–5335. doi: 10.1093/nar/gkab281

Figure 1.

Figure 1.

Global mapping of FinO targets Salmonella enterica SL1344. (A) Schematic representation of genetic elements present in S.enterica SL1344, the chromosome and the three plasmids pSLT, pCol1B9 and pRSF1010. Below, schematic representation of pSLT plasmid encoded protein FinO, chromosome encoded protein ProQ and pCol1B9 plasmid encoded FopA. (B) Upper panel, detection by western blot of immunoprecipitated FinO-3×FLAG and ProQ-3×FLAG. Lower panels, direct detection by northern blot of co-enriched FinP and SibA sRNAs in ProQ and FinO RNA co-immunoprecipitation samples. (C) Volcano plot of RNA transcripts enriched by RIP-seq of FinO-3×FLAG (left panel) and ProQ-3×FLAG (right panel). RNA coimmunoprecipitated with tagged FinO-3×FLAG or ProQ-3×FLAG was compared to the non-tagged WT strain to determine enrichment factors. (D) Pie chart representing the read distribution of sRNAs enriched by FinO and ProQ on RIP-seq samples from panel C.