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. 2021 May 3;49(9):5106–5123. doi: 10.1093/nar/gkab313

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Histone acetylation and gene expression uncoupled at PS and RS stages. (A) Heat map of H3K9ac binding signal in wild type and Stra8-cre/Hdac3^fl/- from –5 to +5 kb surrounding the TSS of genes at the pachytene spermatocyte and round spermatid stage. ChIP-seq with antibody against H3K9ac was performed in pachytene spermatocytes (PS) and round spermatids (RS) isolated from adult wild type and Stra8-cre/Hdac3^fl/–. (B) Heat map of H3K27ac binding signal in wild type and Stra8-cre/Hdac3^fl/- from –5 to +5 kb surrounding the TSS of genes. ChIP-seq with antibody against H3K27ac was performed in pachytene spermatocytes (PS) and round spermatids (RS) isolated from adult wild type and Stra8-cre/Hdac3^fl/–. (C, D) Average H3K9ac (C) or H3K27ac (D) ChIP signals of Stra8-cre/Hdac3^fl/– PS and RS versus wild-type PS and RS within –5 kb/+5 kb of TSS. CPM, counts per million. (E, F) Average H3K9ac or H3K27ac profiles at upregulated (red), unchanged (gray) and downregulated (blue) genes in Stra8-cre/Hdac3^fl/– versus their wild-types at PS stage. CPM, counts per million. (G) Genome browser tracks depicting reads accumulation of H3K9ac and H3K27ac on representative meiotic gene Rad51 and postmeiotic haploid gene Sox30 in WT and Stra8-cre/Hdac3^fl/– at PS and RS stages. Y axis scales in brackets represent reads per million.