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. 2021 May 20;16(5):e0251632. doi: 10.1371/journal.pone.0251632

Fig 3. Diazepam inhibits mouse and human T cell proliferation via different pathways.

Fig 3

Splenocytes and PBMC were treated with α-CD3, 50 μM GABA, 100 μM diazepam, and a cocktail of GABA(A)R inhibitors consisting of 100 μM BMI and 50 μM TPMPA. The reduction in proliferation under each condition as compared to a positive control of α-CD3 alone was assessed by flow cytometry following 48 hours of treatment for splenocytes, and 96 hours of treatment in PBMC. A. Treatment with the GABA(A)R inhibitor cocktail is able to rescue CD8+ and CD4+ T cell proliferation in murine T cells. B. Treatment cannot rescue proliferation in humans T cells. Data shown is from one representative experiment, with error bars (SD). Differences between groups were assessed by one-way ANOVA. * = p<0.05. ** = p<0.01. *** = p<0.001. **** = p<0.0001.