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. 2020 Dec 7;33(2):322–337. doi: 10.1093/plcell/koaa029

Figure 1.

Figure 1

Overexpression of engineered EIN2C–EIN3DB leads to a partial constitutive ethylene-responsive phenotype. (A) Diagrams showing the fusion of EIN2C with EIN3DB. Green indicates the proline-rich region; blue indicates the second and third basic motifs in EIN3DB. (B) The ratio of adult EIN2C–EIN3DB and ein3-1 eil1-1 EIN2C–EIN3DB plants that displayed a dwarf phenotype. Eight-week-old plants were grown under 16 h light and 8 h dark before investigation. Different letters indicate significant differences, determined by hypergeometric probability (P <0.05). (C) The phenotypes of 3-day-old Col-0 and ein3-1 eil1-1 seedlings that express EIN2C–EIN3DB or EIN2C–EIN3DBK245N. Seedlings were grown on MS medium with or without 10-μM ACC. Bar indicates 2.5 mm. (D and E) Lengths of (D) hypocotyls and (E) roots of 3-day-old etiolated seedlings of the indicated phenotypes. Data are means ± SD of at least 30 seedlings. Different letters indicate significant differences, determined using one-way ANOVA (Analysis of Variation) Scheffe method (P < 0.05). (F) Venn diagrams showing ethylene-regulated genes that are differentially expressed in ein3-1 eil1-1 EIN2C–EIN3DB or ein3-1 eil1-1 EIN2C–EIN3DBK245N seedlings compared to Col-0. Total RNA was prepared from 3-day-old etiolated seedlings treated with air. Differentially expressed genes were identified by reads per kilobase per million reads (RPKM) ≥1 with twofold change and q ≤ 0.05.