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. 2020 Dec 7;33(2):322–337. doi: 10.1093/plcell/koaa029

Figure 6.

Figure 6

Histone acetylation induced by EIN2 does not depend on EIN3. (A) The phenotypes of 3-day-old dCas9-EIN2-C/ein3-1 eil1-1, gETR2-dCas9-EIN2-C ein3-1 eil1-1, and gEBF2-dCas9-EIN2-C ein3-1 eil1-1 transgenic etiolated seedlings grown on medium with or without 10-μM ACC. Bar indicates 2.5 mm. (B and C) Lengths of hypocotyls (B) and roots (C) of 3-day-old etiolated seedlings grown with or without 10-μM ACC. Values are means ± sd of at least 30 seedlings. Different letters indicate significant differences determined using one-way ANOVA Scheffe method (P < 0.05). (D–E) Levels of (D) H3K14Ac and (E) H3K23Ac in the guide RNA target loci in 3-day-old etiolated seedlings. Data are fold-changes relative to levels in Col-0 etiolated seedlings grown in air. Each ChIP-qPCR or RT-qPCR was repeated at least three times. Values are means ± sd of three biological replicates. Different letters indicate significant differences determined using one-way ANOVA Scheffe method (P <0.05).