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. 2021 Jan 13;33(3):714–734. doi: 10.1093/plcell/koaa044

Figure 1.

Figure 1

Accumulation of an NHP-hexoside following treatment with resistance enhancers is dependent on UGT76B1. A–C, Pip, NHP, and m/z 308.1346 content in rosette leaves of 5-week-old Wt, ugt76b1, and a UGT76B1-OE line 48 h after watering with control solution or 1 mM dl-Pip. Rosette leaves were harvested, extracted, and analyzed by LC–MS. Bars are means ± sd, n = 4; significant differences are indicated by letters according to one-way ANOVA (P.adj. < 0.05). D–F, Detection of Pip, NHP, and m/z 308.1346 in leaf extracts of 3-week-old plants sprayed with a control solution or 1 mM BTH. Leaves were harvested 48 h after the treatment and analyzed by LC–MS. Bars are means ± sd, n = 4; significant differences are indicated by letters according to one-way ANOVA (P.adj. < 0.05). G, MS/MS fragmentation of m/z 308.1346 ± 0.005 identified in plant extracts. H, Detection of m/z 308.1346 in extracts of Wt Col and of ugt76b1, fmo1, and fmo1 ugt76b1 mutant lines using LC–MS.