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UGT76B1 glucosylates NHP in vitro. A, LC–MS separation of the in vitro reaction of UGT76B1 (blue curve; control in stippled red) with NHP and UDP glucose producing an NHP glucoside with m/z 308.1346, which shows the correct MS/MS fragmentation pattern (Supplemental Figure S1C). B, NHP inhibits the UGT76B1-dependent SA glucosylation. Means ± sd, n = 3. Additional mutual inhibitions of the UGT76B1 substrates SA, NHP, and ILA are shown in Supplemental Figure S5.
