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. 2021 Jan 13;33(3):714–734. doi: 10.1093/plcell/koaa044

Figure 6.

Figure 6

Enhanced accumulation of defense-related transcripts and metabolites by the ugt76b1 mutant is dependent on FMO1. A, RT-qPCR analysis of FMO1 transcript abundance of leaves of 4-week-old Wt and ugt76b1 plants grown under short-day conditions; the normalized relative quantity was determined based on the UBQ5 and S16 internal standards; bars show means ± sd, n = 4; differences between genotypes were analyzed by Welch two-sample t test; *P < 0.05. B–F, Pip- and SA-related metabolites were determined using leaf extracts of 4-week-old Wt, ugt76b1, fmo1, and fmo1 ugt76b1 plants grown under short-day conditions using LC–MS. Bars show means ± sd, n = 4; significant differences between genotypes were analyzed by Welch two-sample t test; *P < 0.05 (C) and by one-way ANOVA with post hoc Lincon test as indicated by letters (P.adj. < 0.05) (B and D–F).