Figure 2.
T-DNA insertion mutants of AtDP1/AtDIR12. (A) Schematic representation of AtDP1/AtDIR12 with two T-DNA insertion mutants used in this study. The thick line indicates the coding region and the thinner line indicates the 5′- and 3′-untranslated regions. AtDP1/AtDIR12 has no introns. White and black triangles show the left and right borders, respectively. Numbers indicate the position of the T-DNA insertion. LB, left border; RB, right border. (B) RT-PCR analysis of transcripts in wild-type (WT, Col-0) and two independent homozygous mutant lines (atdp1-1 and atdp1-2). C, Scanning electron micrograph of epidermal cells from a dry seed of WT (Col-0) and the homozygous atdp1-1 mutant. Scale bars = 100 µm.