Figure 8.

Growth of H.a. Noco2 and P.s.m. on the distal leaves of wild-type (Col-0), ugt76b1-1, FMO1-3D, and fmo1-1 plants. A, Three-week-old plants were first infiltrated with P.s.m. (OD₆₀₀ = 0.001) or 10 mM MgCl₂ (mock) on two primary leaves and sprayed with H.a. Noco 2 spores (5 × 10⁴ spores/mL) 2 days later. Infections on systemic leaves were scored 7 days after inoculation as described previously (Zhang et al., 2010). A total of 15 plants were scored for each treatment. Disease rating scores are as follows: 0, no conidiophores on the plants; 1, one leaf was infected with no >5 conidiophores; 2, one leaf was infected with >5 conidiophores; 3, two leaves were infected but no >5 conidiophores on each infected leaf; 4, two leaves were infected with >5 conidiophores on each infected leaf; 5, >2 leaves were infected with >5 conidiophores. Similar results were obtained in three independent experiments. (B) Four-week-old plants were first infiltrated with P.s.m. (OD₆₀₀ = 0.001) or 10 mM MgCl₂ (mock) on two primary leaves. Two days later, two upper leaves were challenged with P.s.m. (OD₆₀₀ = 0.001). Infections on systemic leaves were scored directly after (0 dpi) and 3 days post-inoculation (3 dpi). Error bars represent standard deviation. Letters indicate statistical differences (P < 0.05, one-way ANOVA; n = 7–8 biological replicates each representing a single infected plant). The results were confirmed by a second independent experiment.