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. 2020 Dec 4;33(2):290–305. doi: 10.1093/plcell/koaa023

Figure 4.

Figure 4

Coupled activity of CYP76M8 with CYP99A2 or CYP99A3. (A) and (B) GC–MS chromatogram of extract from E. coli engineered to produce syn-pimaradiene and co-expressing CYP76M8 and (A) CYP99A2 or (B) CYP99A3, along with the requisite CPR redox partner. (C) GC–MS chromatogram of novel product after purification. Peaks numbered as described in the text and shown in the figure (note that -Me indicates methylated carboxylate). (D) Mass spectrum of 8. (E) Mass spectrum of 9, along with the corresponding chemical structure, as determined by NMR.