TABLE 1.
Different techniques for LSD diagnosis
| Techniques | Purposes | |||||
|---|---|---|---|---|---|---|
| Animals freedom from infection | Animal freedom from infection previous to movement | Contribution in eradication policies | Confirmation of clinical cases | Prevalence of infection surveillance | Immune status post‐vaccination | |
| Identification of agent | ||||||
| Virus isolation | + | ++ | + | +++ | + | ‐ |
| PCR | ++ | +++ | ++ | +++ | + | ‐ |
| Electron microscopy | ‐ | ‐ | ‐ | + | ‐ | ‐ |
| Immune response detection | ||||||
| Virus neutralization | ++ | ++ | ++ | ++ | ++ | ++ |
| Electron microscopy | + | + | + | + | + | + |
−: not appropriate for the purpose; +: may be used in some situations, but its application is limited by some factors such as reliability, cost, etc.; ++: appropriate method; +++: recommended method.
IFAT indicates Indirect Fluorescent Antibody Test; and PCR, polymerase chain reaction.