TABLE 1.
List of primers used for quantitative real‐time PCR analysis of the relative quantity of transcripts in 1‐day‐old piglets and adult pigs of the Berkshire and Jeju native pig breeds
| Gene Name | Primer sequences (5′−3′) | Tm | Product size | Gene bank ID |
|---|---|---|---|---|
| MyoD |
F: TGCAAACGCAAGACCACTAA R: GCTGATTCGGGTTGCTAGAC |
55 °C | 127 | NM_001002824.1 |
| Pax7 |
F: GGCAGAGGATCTTGGAGACA R: TGGGTGGGGTTTTCATCAAT |
55 °C | 144 | AY653213.1 |
| Myf6 |
F: ATCTTGAGGGTGCGGATTTC R: CAATGTTTGTCCCTCCTTCCT |
62 °C | 108 | XM_003481764 |
| Myf5 |
F: CCGACACAGCTTGTGGAATA R: GCCAATCAACTGATGGCTTT |
55 °C | 128 | XM_001924362.2 |
| MyBPH |
F: AGTGCAGAAGGCAGACAAA R: AAGACCCGGAAGGAGTAAGA |
62 °C | 117 | NC_010451 |
| β‐actin |
F: GACATCCGCAAGGACCTCTA R: ACACGGAGTACTTGCGCTCT |
60 °C | 157 | XM_003124280 |
Direction: F means forward, and R means reverse