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Sperm chromatin structure assay (SCSA) immediately after thawing. DNA Fragmentation Index (%DFI) and % of spermatozoa with high green fluorescence indicating immature spermatozoa. TEY = Tris egg yolk, L2 = extender with lecithin type II‐S, L4 = extender with lecithin type IV‐S. Different letters within parameter indicate significant difference (p < 0.05). Mean ± SE. N = 8 dogs
