Video 2. Representative example of multiphoton microscopy processed with Imaris software of a femur from a Lyve1^eGFP mouse treated with LPS at 1 hr after administration (zeitgeber time 5).

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Bone tissue is identified as second-harmonic signal (blue). Imaris software was used to measure distance between DiI-labeled cells and green fluorescent protein-positive lymphatic vessels using 3D images.