Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Gene (Mus musculus) | Draxin | Mus musculus genome resource | 70433 | |
Strain, strain background (Mus musculus) | BTBR T + Itpr3tf/J | The Jackson Laboratory | 002282 | |
Strain, strain background (Mus musculus) | C57Bl/6J | The Jackson Laboratory | 000664 | |
Cell line (Homo sapiens) | Human embryonic kidney (HEK) 293T | ATCC | RRID:CVCL_0045 | ATCC Cat# CRL-1573, obtained via the University of Queensland |
Antibody | Sheep polyclonal anti-DRAXIN | R&D Systems | AF6149, RRID:AB_10640005 | ‘(1:250)’ |
Antibody | Mouse monoclonal anti-human KI67 | BD Pharmingen | 550609, RRID:AB_393778 | ‘(1:500)’ |
Antibody | Mouse monoclonal anti-GAP43 | Millipore | MAB347, RRID:AB_94881 | ‘(1:500)’ |
Antibody | Rabbit polyclonal anti-GFP | Thermo Fisher Scientific | A-6455, RRID:AB_221570 | ‘(1:1000)’ |
Antibody | Rabbit polyclonal anti-GFAP | Dako | Z0334, RRID:AB_10013382 | ‘(1:500)’ |
Antibody | Mouse monoclonal anti-Glast (EAAT1) | Abcam | Ab49643, RRID:AB_869830 | ‘(1:500)’ |
Antibody | Rabbit polyclonal anti-Glast (EAAT1) | Abcam | Ab416, RRID:AB_304334 | ‘(1:250)’ |
Antibody | Chicken polyclonal anti-Laminin | LS-Bio | C96142, RRID:AB_2033342 | ‘(1:500)’ |
Antibody | Rabbit polyclonal anti-Laminin (pan-Laminin) | Sigma | L9393, RRID:AB_477163 | ‘(1:500)’ |
Antibody | Rat monoclonal anti-Nestin (NES) | Developmental Studies Hybridoma Bank | AB 2235915, RRID:AB_2235915 | ‘(1:50)’ |
Antibody | Rabbit polyclonal anti-SOX9 | Merck | AB5535, RRID:AB_2239761 | ‘(1:500)’ |
Antibody | Goat anti-β-ACTIN | SCIGEN | AB0145-200, N/A | ‘(1:1000)’ |
Recombinant DNA reagent | pPBCAG-IRES-GFP | Chen et al., 2020 | ||
Recombinant DNA reagent | pGEMT | Promega | A1360 | |
Sequence-based reagent | Draxin_riboprobe_F | Allen Brain Atlas | CAGGGAGGTTTAGGACAAACAG | |
Sequence-based reagent | Draxin_riboprobe_R | Allen Brain Atlas | TGTAGGAGCTGAGGGAAAGAAG | |
Sequence-based reagent | Draxin_CDS_F | This paper | GAATTCGACAGGGAGAGCCAATG | |
Sequence-based reagent | Draxin_CDS_R | This paper | GCGGCCGCGTACTGGGCGTACACCTGCT | |
Sequence-based reagent | Chromosome 4, SNP rs6397070 forward | This paper | TTTATGGCTGGGGACTTCAG | |
Sequence-based reagent | Chromosome 4, SNP rs6397070 reverse | This paper | CGAATCCAAAGCTCTCTTGC | |
Sequence-based reagent | Chromosome 9, SNP rs29890894, forward | This paper | AGCTTGGTGGCATCCATATC | |
Sequence-based reagent | Chromosome 9, SNP rs29890894, reverse | This paper | GCACTCTCCCTACTGCTTGG | |
Sequence-based reagent | Chromosome 15, SNP rs31781085 forward | This paper | GATCGTTGCAGTGACCACAC | |
Sequence-based reagent | Chromosome 15, SNP rs31781085 reverse | This paper | GCTGATTGGCAGGTTCTGAT | |
Sequence-based reagent | Draxin allele genotyping, wildtype forward | This paper | AGACGGTCCCTGCGTCTC | |
Sequence-based reagent | Draxin allele genotyping, mutant forward | This paper | GTCGCAGACGGTCCCTTG | |
Sequence-based reagent | Draxin allele genotyping, common reverse | This paper | AGGCTTCCCAGATGACACTC | |
Commercial assay, kit | Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye | Invitrogen | C10337 | |
Commercial assay, kit | Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 555 dye | Invitrogen | C10338 | |
Software, algorithm | Fiji | Fiji | RRID:SCR_002285 | |
Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | |
Software, algorithm | Imaris | Bitplane | N/A | |
Other | 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) | Invitrogen | D1306 | ‘(1:750)’ |