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. 2021 May 6;12:663731. doi: 10.3389/fmicb.2021.663731

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Copyright © 2021 Chang, Zhang, Sun, Li, Lin, Yang, Webber and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Formation of a circular intermediate by oqxABR operon. (A) Comparison between genetic structure of transposon Tn6010 located in pOLA52 (accession no. NC_010378) and the oqxABR operon positive strains. (B) The circular form of Tn6010 and the approximate locations of the reverse primers oqx-IF and oqx-IR (line arrows). (C) Gel electrophoresis of PCR amplicons corresponding to the circular intermediate of Tn6010, detectable in oqxABR-positive strains using the reverse primers oqx-IF and oqx-IR. Primers are listed in Supplementary Table 1. NC, Negative Control.