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. 2021 Feb 26;101(6):680–689. doi: 10.1038/s41374-021-00538-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A RT-PCR analysis of TRPV1 expression using gene specific primers revealed TRPV1 in HCK (lane 1) and HCEC-12 (positive control, lane 4). Template-free negative controls (lane 2: template replaced with H₂O, lane 3: w/o reverse transcriptase in the reverse transcription reaction) gave no bands. M = 100 bp plus DNA ladder. B 20 µmol/l CAP induced an increase in Ca²⁺ influx (n = 21) in HCK. C Same experiment as shown in B, but in the presence of AMG 9810. AMG 9810 (10 µmol/l) clearly suppressed the CAP-induced Ca²⁺ increase even partially below the base line level (n = 23). D Summary of the experiments with CAP and AMG 9810 in HCK. The asterisks (*) designate significant increases in [Ca²⁺]_i with CAP (t = 300 s; n = 21; p < 0.005; paired tested) compared to control (t = 100 s). The hashtags (#) indicate statistically significant differences in fluorescence ratios between CAP with and without AMG 9810 (t = 300 s; n = 21–23; p < 0.005; nonpaired tested).