Table 3.
Phenotypic characteristics which distinguish isolate PRKS01-29T from the type strains of closely related species classified in the Streptomyces violaceusniger clade
| Characteristics | Strains | ||||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | |
| API-ZYM tests | |||||||
| Esterase (C4) | + | − | − | + | + | + | + |
| α-Fucosidase | − | − | − | − | + | − | + |
|
α- and β-Galactosidase, α-mannosidase, trypsin |
+ | + | + | + | + | − | + |
| β-Glucuronidase | − | − | − | + | − | − | − |
| α-Glucosidase | + | − | − | − | − | − | − |
| β-Glucosidase | − | − | + | + | − | − | − |
| Lipase (C14) | + | − | + | − | − | + | − |
| Biochemical tests | |||||||
| Nitrate reduction | − | − | − | + | − | + | + |
| Degradation tests | |||||||
| Adenine (0.5%, w/v) | + | + | − | + | + | + | + |
| Aesculin (0.1%, w/v) | − | − | + | + | + | − | + |
| Allantoin (0.5%,w/v) | + | − | + | + | − | + | − |
| Arbutin (0.5%, w/v) | − | + | + | + | − | + | − |
| Casein (1%, w/v) | + | − | + | + | + | − | + |
| Guanine (0.3%, w/v) | − | − | − | + | − | + | − |
| Starch (0.1%, w/v) | + | + | + | + | + | − | − |
| Tween 20 (1%, v/v) | + | − | + | + | + | − | − |
| Uric acid (0.4%, w/v) | + | − | + | + | + | + | − |
| Xylan (0.4%, w/v) | + | + | + | + | − | + | + |
| Tolerance tests | |||||||
| Growth in presence of 7% w/v, NaCl | + | − | − | + | + | + | − |
| Growth at pH 9.0 | − | − | + | – | + | + | − |
| Growth at 45 °C | + | + | − | + | − | − | + |
Strains: 1. Isolate PRKS01-29T., 2. S. albiflaviniger DSM 14598T., 3. S. iranensis DSM 41954T., 4. S. javensis DSM 41764T., 5. S. rapamycinicus NRRL 5491T., 6. S. rhizosphaericus NRRL B-24304T., 7. S. violaceusniger DSM 40563T
All of the strains were positive for acid and alkaline phosphatases, α-chymotrypsin, cystine, leucine and valine arylamidases, esterase (C4), esterase lipase (C8), N-acetyl-β-glucoronidase and naphthol-AS-BI-phosphohydrolase (API-ZYM tests), hydrolysed urea and degraded hypoxanthine (0.4%, w/v), Tweens 40, 60 and 80 (all 1%, v/v) and L-tyrosine (0.4%, w/v), but not chitin (1%, w/v), elastin (0.3%, w/v), tributyrin (0.1%, w/v) or xanthine (0.4%, w/v)
+ positive., − negative, n.d. not determined