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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Liver Int. 2021 Feb 16;41(6):1305–1319. doi: 10.1111/liv.14811

Figure 3. The maternal HF diet disrupted folic acid metabolism in offspring, while offspring of H9N diet mothers had normal folate metabolism.

Figure 3.

3A-B. Gene expression levels of Shmt1, Shmt2, Mthfr, Mthfd1, Mthfd2, and Dhfr were measured by real-time PCR in male (A) and female (B) offspring livers. 3C. Betaine, choline and dimethylglycine (DMG) concentrations were detected via HPLC analysis. 3D. The expression levels of genes encoding methyltransferases for betaine metabolites and methyl group delivery were measured by real-time PCR in male offspring livers. 3E. Methionine and SAH contents were detected via high-resolution UPLC-MS/MS. 3F. Gene expression levels of Mtr were measured by real-time PCR in male and female offspring livers. Data are mean ± SE, n=5. * indicates P<0.05 vs. NF group. # indicates P<0.05 vs. the REF group.