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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Cancer Res. 2021 Feb 1;81(9):2275–2288. doi: 10.1158/0008-5472.CAN-20-1541

Figure 1.

Figure 1.

APC status impacts serine synthesis gene expression in murine intestinal tumors. (A) The expression of Phgdh, Psat1 and Psph was compared in ileal polyps from ApcMin/+ mice vs. normal ileal mucosa from WT mice by qRT-PCR. Gene expression in polyps was calculated relative to normal mucosa (n=5 per group). **P<0.01 compared to normal tissue for each gene. (B) Expression levels of Phgdh, Psat1 and Psph were determined by RNA seq in colonic tissue from shRen mice given doxycycline for 15 weeks (shRen + dox), shApc mice given doxycycline for 15 weeks (shApc + dox) and shApc mice 4 days following doxycycline removal (shApc off dox) (n=4 per group). *P<0.05; ***P<0.001 as indicated. (C) Expression levels of Phgdh, Psat1 and Psph were determined by RNA seq in colonic tissue from shApc/KrasG12D mice given doxycycline for 15 weeks and shown along with data found in panel B from the shRen + dox and shApc + dox groups (n=4 per group). *P<0.05; **P<0.01; ***P<0.001 compared to the shRen group for each gene as indicated.