Figure 4. Overexpression of miR-31 promotes metastasis in large cell, small cell, and squamous cell lung carcinoma.
H661 (LCLC), H82 (SCLC), and Calu-1 (SQCC) cells were infected with miR-31–encoded retrovirus (miR-31) or empty retrovirus (vector) control. Cells were injected subcutaneously into the flanks of immunocompromised mice. Mice were evaluated for spontaneous metastases at the time of necropsy. (A-C) Quantification of spontaneous metastases per section to the lung (A, C) and liver (B). Representative images shown, 20X. Number (n) of mice indicated.