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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: Cancer Res. 2021 Feb 15;81(8):1965–1976. doi: 10.1158/0008-5472.CAN-20-3213

Figure 4. Tet2 deletion enhances the transcription of tumor-suppressive genes in TILs.

Figure 4.

A. The experimental design for comparative RNA-seq and ATAC-seq analyses.

B. Venn diagrams showing WT- and Tet2-specific differentially expressed genes (DEGs) identified between Day 0 and Day 3 adoptively transferred TILs (WT vs Tet2KO).

C. GOplot illustrating the top 5 genes in the top three categories from the GSEA analysis of WT- and Tet2-specific DEGs. The left side of the circle displays the DEGs and color represents the log2 fold change (logFC). Red, upregulated genes; Blue, downregulated genes. The right side of the circle shows the GSEA categories.

D. Real-time quantitative PCR validation of selected DEGs annotated as regulators of immune response.

E-F. The UCSC genome browser view of RNA-seq data for representative genes that are involved in CD8+ T cell immunity and are up-regulated in the Tet2KO group.