Figure 1.

Multiphoton‐guided creation of organ‐specific vascular structures at anatomic scale. a) Hydrogel photoablation. Images shown following perfusion of beads (magenta). b) Schematic of ablation and perfusion process. c) Two‐photon images of a human alveolus (left), and an engineered alveolar‐capillary unit after bead perfusion of capillary (red) and alveolar (blue) compartments (right). d) Recreation of mouse brain microvasculature. i) Schematic of using in vivo imaging data as a mask for ablation. ii) 3D microvascular image traced to isolate an arteriole (red), venule (blue), and capillaries (yellow). iii) Projection images of a replica microvascular unit ablated into collagen and perfused with beads (red). Orthogonal projections and capillary cross‐section shown. Arrows delineate flow from arteriole (A), to capillaries (C), and venule (V).