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. 2021 Feb 15;10(10):2100031. doi: 10.1002/adhm.202100031

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© 2021 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Creation of heterogeneous, hierarchical, and multicellular vasculature. a) Heterogeneous vasculature fabrication. Images 24 h post‐ablation (bottom) show a continuous vessel containing heterogeneous endothelial cell populations. b) Hierarchical vasculature creation. Image 72 h after HUVEC seeding (bottom) show a muscularized arteriole (A) and venule (V) connected by a microvascular network (N) lined by a HUVEC monolayer. c) Vascularization of cellularized tissue. Projection image following ablation of a “spiral” lumen into collagen containing HS27a‐mCherry stromal cells (shown here in green) followed by seeding of HUVECs. Inset shows magnification of stromal cell/HUVEC association.