Figure 5. Crosslinks mapped to cryo-EM structural model of the CYP102A1 homodimer in Closed (A and C) and Open II (B and C) conformations.
Since the dimer is not symmetrical, we arbitrary labeled one monomer α (light grey) and the other β (dark grey). The heme-containing oxygenase (BMP) and flavin-containing reductase (BMR) domains of each monomer are labeled accordingly. Cofactors and crosslinks are colored as in Figure 3. 27Å scale bar indicates the accepted Cα-Cα distance restraint for the DSBU crosslinker. Crosslinks #1–8 with the exception of #6 from Table 5 are shown in A and B. Crosslinks #9–19 from Table 5 are shown in C and D.