Fig. 7.

Effects of tested urolithins and respective glucuronides at the concentration of 40 μM on f-MLP-induced β-glucuronidase release from human primary neutrophhils. After isolation, neutrophils were resuspended in HBSS with iso-urolithin A, urolithin A and B (iUA, UA, UB) and their respective glucuronides (GiUA, GUA, GUB) at the concentration of 40 μM primed with cytochalasin B (10 μM) for 5 min and then stimulated with f-MLP (1 μM) for 10 min. Statistical significance: *p < 0.05, **p < 0.001 decrease versus stimulated control (Dunnettʼs post hoc test); fMLP—stimulated control; NST—non-stimulated control. Genist-genistein at the concentration of 40 μM (positive control). Data were expressed as mean ± SD of three separate experiments performed with cells isolated from independent donors. Mean ± SD and p values are provided in Supplementary material Table S7