Figure 4.
The formation of platelet-tumor cell aggregates is necessary for NET-mediated capture. (A) Representative flow cytometry plots of NET-captured tumor cells in an in vitro co-culture setting in the presence and absence of platelets. (B) Time course of tumor cell adhesion to NETs in the presence and absence of platelets under dynamic conditions. Blue: MC38; green: NETs; red: Platelet. (C and D) Quantitation of tumor cells that adhered to NETs under dynamic and static conditions. Quantitation was performed by counting the number of cells from 6 images per experiment (×20). (E) The migration rates of MC38 towards NETs in the presence and absence of platelets were measured by transwell migration assays. The number of migrated cells were counted in 6 images per experiment (×20). Data from three or more independent experiments are presented as mean ± SEM. ns: not significant, *P<0.05, **P<0.01, ***P<0.001 and ****P < 0.0001.