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. Author manuscript; available in PMC: 2022 May 17.
Published in final edited form as: Dev Cell. 2021 Apr 19;56(10):1437–1451.e3. doi: 10.1016/j.devcel.2021.03.026

Figure 5. Pu.1 stably expressing cells are differentiation-defective and inhibit adipogenesis of nearby preadipocytes in vivo.

Figure 5.

(a) Adipocyte differentiation of 3T3-L1 cells cultured in conditioned media from ARC or Pu.1 KD ARC. (Left) Oil Red O staining, (Right) RT-qPCR for Sox9, C/ebpβ, Pparγ, and Fabp4 (n=6).

(b) Schematic of the implantation experiment (n=4).

(c) Whole-mount staining of implants with LipidTox (Red), DAPI (blue) and GFP (Green).

(d) RT-qPCR for C/ebpβ, C/ebpδ, Pparγ, and Fabp4 (n=8).

Data are represented as mean ± S.D. *≤0.05, **≤0.01, ***≤0.001.