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. 2021 May 20;12:2739. doi: 10.1038/s41467-021-22977-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Localization pattern of GSH1, EDS5, and CAS proteins in CHUP1ox and chup1 plants. The transgenic gsh1 CHUP1ox and gsh1 chup1 plants expressing GSH1-GFP under its own promoter, and CHUP1ox and chup1 plants expressing EDS5-sfGFP and CAS-sfGFP under the CaMV 35S promoter were incubated with Cfio and observed at 2 dpi. DW was used as a control. The chloroplasts were visualized based on chlorophyll autofluorescence. The white arrowheads and arrows indicate melanized appressoria and stromules, respectively. Scale bar, 10 µm. b Quantification of epidermal cells with surface fluorescence of GFP/sfGFP-labeled immune components. Cfio-inoculated cotyledons of the indicated Arabidopsis were analyzed at 2 dpi. A total of 100 cells in contact with the melanized appressorium were observed. As a control, DW was spotted. The means and SE were calculated from three independent plants. Means not sharing the same letter are significantly different (P < 0.05, two-way ANOVA with Tukey’s HSD test).