Fig. 1.

a, b. PRF setting. High (a) and low (b) PRF setting show a better evidence of intra-testicular flow at low setting (white box; a, b). c, d, e Trans-mediastinal testicular vessels. Longitudinal scans of testis with power-Doppler (c), color-Doppler (d) and superb microvascular imaging (SMI) (e) mode show an artery branching from the mediastinum testis and running within the parenchyma. f Mediastinum testis and albuginea. Longitudinal B-mode scan of testis shows a normal homogeneous echotexture of testis with a mediastinum testis (arrow) as a linear echogenic band of fibrofatty tissue; a thin echoic line surrounds the testis and corresponds to the tunica albuginea (arrowheads). g Head of epididymis. Longitudinal B-mode scan of testis with virtual convex field of view shows the head of epididymis (arrows) with a normal homogeneous echotexture. T: testis. h, i Flow of trans-testicular arteries. Longitudinal scan of testis shows normal testicular arteries representation at color-Doppler mode (h) with low-impedance waveform with large amount of end diastolic flow at pulse-Doppler mode as well as low resistance pattern (i). l, m, n Normal testicular anatomy at CEUS. Longitudinal scans of testis after contrast agent administration show: Testicular arteries enhance first (l) followed by a complete fill-in of the parenchyma and a progressive late washout (n). (white box: time of the scans; l, m, n). o Normal penis anatomy. Transverse B-mode ventral scans of the penis at the basis shows two hypoechoic images (C) corresponding to the corpora cavernosa with a thin echoic line that surrounds them and corresponds to the tunica albuginea (arrowheads); the corpus spongiosum (S) is adjacent to the corpora cavernosa. p Cavernous artery. Longitudinal, ventral scan of the penis, with color-Doppler and pulsed mode shows a normal flow pattern of the cavernous artery (arrow) in a flaccid penis