Figure 1.

Comparison of MOG-IgG negative and positive samples by live cell-based assay immunofluorescence (CBA-IF). Representative fluorescence image of (A) negative and (B) positive samples. The green fluorescence DyLight 488 is an indicator of bound human serum MOG antibody. The blue fluorescence is a nuclear DAPI marker. Images were obtained using a confocal microscope with a 63× objective lens. (C) Represents the quantification of CBA-IF binding scores for negative and positive samples. The cut-off value for seropositivity (1:128 dilution) was represented by the dotted line. The titers of positive samples range from 1:128 to 1:65,556.