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. 2021 May 7;12:642272. doi: 10.3389/fimmu.2021.642272

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Copyright © 2021 Marchionatti, Hansel, Avila and Sato

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scatter plots graphs of live cell-based assay flow cytometry (CBA-FC) analysis. (A) Representative flow cytometry strategy gating for HEK293 cells. The gate of flow cytometry data was based on forward scatter (FSC) versus side scatter (SSC), using a FlowJo™. Representative flow plots of (B) MOG-IgG^-, and (C) MOG-IgG⁺ samples. The X-axis represents the MOG-EGFP transfection marker (488 nm) and the Y-axis represents the secondary anti-human Fc-IgG DyLight 650 nm antibody binding.