Table 1.
Cryo-EM data collection, refinement, and validation statistics.
| YnaI (EMDB-20862) (PDB 6URT) | |
|---|---|
| Data collection and processing | |
| Magnification | 45,000 |
| Voltage (kV) | 200 |
| Electron exposure (e–/Å2) | 64.3 |
| Defocus range (μm) | −0.1 to −2.5 |
| Pixel size (Å) | 0.864 |
| Symmetry imposed | C7 |
| Initial particle images (no.) | ~800,000 |
| Final particle images (no.) | 177,979 |
| Map resolution (Å) | 3.3 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.0–4.6 |
| Refinement | |
| Initial model used (PDB code) | N/A |
| Model resolution (Å) | 3.3 |
| FSC threshold | 0.5 |
| Model resolution range (Å) | |
| Map sharpening B factor (Å2) | −129.9 |
| Model composition | |
| Non-hydrogen atoms | 17920 |
| Protein residues | 2317 |
| Ligands | |
| B factors (Å2) | |
| Protein | −120 |
| Ligand | |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.009 |
| Bond angles (°) | 1.050 |
| Validation | |
| MolProbity score | 2.85 |
| Clashscore | 6.16 |
| Poor rotamers (%) | 0.7 |
| Ramachandran plot | |
| Favored (%) | 91.14 |
| Allowed (%) | 8.86 |
| Disallowed (%) | 0 |