Figure 3.

Yeast interfering RNA larvicide technology can be used for scaled production of males. Yeast larvicide #478 does not significantly impact male mating capacity [(a), p > 0.05, Student’s t-test], the number of eggs laid by females that mated with these males [(b), p > 0.05, Student’s t-test], or the percentage of larvae that hatched from these eggs [(b), p > 0.05, Student’s t-test]; results were compiled from 41 matings with #478-treated males and 72 matings with males treated with control interfering RNA yeast). Incorporation of the yeast larvicide into a larval diet used for mass-rearing (MR; n = 1200 total larvae per treatment) resulted in significant female mortality [(c), *** = p < 0.001, Chi-square] with no significant impact on male survival [(c), p > 0.05, Chi-square] or fitness [(d), p > 0.05, Student’s t-test; n = 83 control diet male wings, n = 40 #478-treated male wings]. Error bars denote SEM in all panels.