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. 2021 May 20;12:2979. doi: 10.1038/s41467-021-23249-y

Fig. 5. Whole-body fluorescent imaging of Medaka larvae.

Fig. 5

a Three-dimensional reconstructed view of the lymphatic and blood vessels in the whole body of a 2 wph larva. Green shows the EGFP signals from the FLT4-EGFP transgenic strain, and red shows the dextran-conjugated rhodamine signals from the blood vessel. The yellow rectangular shows a magnification view at the body trunk. Exposure time is 100 ms, z-interval is 2 μm, and xy 1024 × 1024 pixels. n = 3 biologically independent larvae are subjected to the analysis. b Three-dimensional reconstructed view (left) and a sliced view (right) of the whole brain of the Kif5Aa-EGFP strain larva. Exposure time, z-interval, and xy are 100 ms, 2 μm, and 1024 × 1024 pixels, respectively. n = 5 biologically independent larvae are subjected to the analysis. Abbreviations; DA dorsal aorta, DL dorsal lymphatic, DLAV dorsal longitudinal anastomotic vessel, LL lateral lymphatic, PCV posterior cardinal vein, SL spinal lymphatic.