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. 2021 May 20;12:2979. doi: 10.1038/s41467-021-23249-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Bright field view of the 3 dpf embryos of the β-actin-DsRed2 strain after the phototoxicity experiment. Control indicates a gel-embedded embryo, which does not show significant damage. For the ablation experiment, the time-course 4D imaging was performed in 5-min intervals with 200 ms exposure/slice with 2 μm steps, 2048 × 2048 pixels, ~600 slices. The arrow shows the air bubble created around the eye. b 3D view of the embryos viewed from the lateral and dorsal side. White arrows indicate strong fluorescence emitted from the retinal pigments. a, b n = 3 biologically independent β-actin-DsRed2 strain embryos are examined for the different beam conditions of phototoxicity experiment.