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. 2021 May 20;12:2979. doi: 10.1038/s41467-021-23249-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Change of the recording mode. To-and-from bidirectional recording mode are implemented. b 4D time-lapse imaging of a GCaMp6f expressed Medaka embryo (1 dpf). Fluorescent signals propagating over the outer layer surrounding whole embryos are indicated. Bottom-right images are two-dimensionally projected maps of the signal using the Mollweide cartography technique. c Plot of the GCaMP6f signal in a small ROI as a function of time. a.u. arbitrary unit. d Fourier analysis of the oscillatory expression of the GCaMP6f signal. a.u., arbitrary unit.