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. 2021 May 20;7:119. doi: 10.1038/s41420-021-00492-2

Fig. 3. MiR-503-3p/DACT2 axis regulates the Wnt/β-catenin signaling pathway, glycolysis, and mitochondrial OXPHOS in BC cells.

Fig. 3

A RT-qPCR measured miR-503-3p and DACT2 expression in CAL-51 cells; B western blot measured DACT2, active-β-catenin, p-β-catenin, Glut1, and LDH-A protein expression in CAL-51 cells; C glucose intake of CAL-51 cells, D oxygen consumption rate of CAL-51 cells; E ATP level in CAL-51 cells; F CAL-51 cell viability; G flow cytometry determined cell cycle distribution. H, I Transwell assay tested migration and invasion ability of CAL-51 cells; J, K tumor volume and weight of nude mice that had been injected with CAL-51 cells; repetitions = 3 in (A–I), n = 6 in (J, K); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; the measurement data were expressed as mean ± standard deviation and compared using Student’s t test.