Fig. 4. Identification of macrophages and exosomes.
A Morphology of PMA-induced macrophages; B identification of macrophages by flow cytometry; C identification of exosomes under a TEM; D western blot measured CD63, CD81, and TSG101 levels; E NTA measured size and concentration of exosomes; F FITC and DIL staining assessed the activity of exosomes entering cells; G, H RT-qPCR measured miR-503-3p expression in macrophages (G) and exosomes (H); repetitions = 3; **P < 0.01; ***P < 0.001; the measurement data were expressed as mean ± standard deviation and compared using Student’s t test.