Figure 2.

OSCC secretome up regulates CCR8 expression in Treg and Teff. (A) Representative dot plots of chemokine receptor expression CXCR3, CCR4, CCR6 and CCR8 in Tregs and Teff after co-culture with control or OSCC secretome. Briefly, sorted memory Tregs and Teff obtained from peripheral blood from 3 healthy donors were pre-activated with anti-CD3/CD28 beads (1:5) in the presence of IL-2 (1000U). After activation, 2x10⁵ Tregs and Teff were co-cultured with secretomes from OSCC or control samples for 48h. After co-culture, cells were stained with Live/Dead dye, chemokine receptor expression and counted with counting beads. (B) Comparison of cell counts between Tregs and Teff co-cultured with OSCC or control secretome. (C) Comparison of CXCR3, CCR4, CCR6 and CCR8 expression between Tregs and Teff co-cultured with OSCC or control secretome. Data are presented as mean ± SEM using bars with scatter dot plots (Unpaired t test). For all statistical tests, ^∗∗p < 0.01 and ^∗p < 0.05 were considered significant.