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. 2021 May 20;12(6):517. doi: 10.1038/s41419-021-03782-w

Fig. 2. shTSP50 affects cell proliferation and aerobic glycolysis.

Fig. 2

A Multiple cancer cells were cultured, harvested and subjected to Western blot analysis for TSP50 detection. B–C shTSP50 was transfected into Huh7 and Bel7402 cells for the gene knockdown and the knockdown efficiency was evaluated. β-actin served as loading control. D, E The Huh7 cell viability and BrdU absorbance were detected in cells after shTSP50 transfection by MTT and BrdU assay. F–M After shTSP50 transfected in Huh7 cells, the ECAR, OCR, glucose consumption, lactate production, LDH activity, ATP levels, G6P levels and 2-PG levels were analyzed. N, O The Bel7402 cell viability and BrdU absorbance were detected in cells after shTSP50 transfection by MTT and BrdU assay. P–W After shTSP50 transfected in Bel7402 cells, the ECAR, OCR, glucose consumption, lactate production, LDH activity, ATP levels, G6P levels and 2-PG levels were analyzed. The aerobic glycolysis values were normalized to protein levels. N = 3 biologically independent replicates. Data were presented as means ± s.d. *P < 0.05, **P < 0.01 as compared to NC group by two-sided Student’s t-test. ns, no significance.